Morphological and genetic characterization of the broad mite Polyphagotarsonemus latus Banks (Acari: Tarsonemidae) from two Mexican populations

Periodo de realización: 1900/01/01 al 2022/01/01

Tipo: Artículo científico

Lugar(es) de estudio: Tapachula de Córdova y Ordoñez, Chis., México
Resumen: "Polyphagotarsonemus latus Banks is considered a polyphagous pest of diverse agriculturaland ornamental crops of global economic significance. Its distribution, host range, variety ofsymptoms, morphological differences, chaetotaxy and several ontogeny reports haveadvanced the idea of P. latus as a species complex. Correct pest identification leads to suitablecontrol treatment. Therefore, the objective of this study was the identification of mitescollected in two different geographic regions in Mexico (Chiapas and Guanajuato) that hadbeen tentatively designated as Polyphagotarsonemus sp. Biometric differences on the morphologyof adults as well the genetic variability were determined by taxonomical and molecular(mitochondrial COI gene) characterization techniques. The identity of the mites fromboth populations was confirmed as P. latus based on taxonomic characters. Biometricparameter variations were found between both populations (70.58% and 53.84% forfemales and males, respectively). The average sequenced fragment size was 447 bp (bothpopulations). A homology search against six P. latus sequences available in the GenBankdatabase revealed that sequence KM580507.1 (from India) shows 83.0–86.41% and99.26–99.52% similarity with the sequences from Guanajuato and Chiapas, respectively.Molecular data indicated a significant divergence between the populations. The genetic distancedemonstrates the population from Chiapas has a higher genetic correspondence(0.010) to the sequence from India (KM580507.1) whereas the population from Guanajuatois more distant (0.191). The genetic distance between the populations of this study andother GenBank sequences is even larger. We consider our results strengthen the hypothesisof P. latus consisting of a species-complex. However, it is essential to extend the studyto other regions including its country of origin (Sri Lanka), and to include ultrastructuralfeatures."

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