Venom characterization of the bark scorpion Centruroides edwardsii (Gervais 1843): Composition, biochemical activities and in vivo toxicity for potential prey
Periodo de realización: 1900/01/01 al 2019/01/01
Tipo: Artículo científico
Lugar(es) de estudio: Tapachula de Córdova y Ordoñez, Chis., México, Lovaina, Bélgica, San José, Costa Rica
Resumen: "In this study, we characterize the venom of Centruroides edwardsii, one of the most abundant scorpions in urban
and rural areas of Costa Rica, in terms of its biochemical constituents and their biological activities. C. edwardsii
venom is rich in peptides but also contains some higher molecular weight protein components. No phospholipase
A2, hemolytic or fibrinogenolytic activities were found, but the presence of proteolytic and hyaluronidase enzymes
was evidenced by zymography.
Venom proteomic analysis indicates the presence of a hyaluronidase, several cysteine-rich secretory proteins,
metalloproteinases and a peptidylglycine a-hydroxylating monooxygenase like-enzyme. It also includes peptides
similar to the Kþ-channel blocker margatoxin, a dominant toxin in the venom of the related scorpion
C. margaritatus. MS and N-terminal sequencing analysis also reveals the presence of Naþ-channel-modulating
peptides with sequence similarity to orthologs present in other scorpion species of the genera Centruroides and
Tityus. We purified the hyaluronidase (which co-eluted with an allergen 5-like CRiSP) and sequenced ~60% of
this enzyme. We also sequenced some venom gland transcripts that include other cysteine-containing peptides
and a Non-Disulfide Bridged Peptide (NDBP).
Our in vivo experiments characterizing the effects on potential predators and prey show that C. edwardsii
venom induces paralysis in several species of arthropods and geckos"