Spider Transcriptomes from Venom Glands: Molecular Diversity of Ion Channel Toxins and Antimicrobial Peptide Transcripts
Cita: Diego, E., Camila Takeno Cologna, Juliana Silva Cassoli, Gerardo A. Corzo. 2016. Spider Transcriptomes from Venom Glands: Molecular Diversity of Ion Channel Toxins and Antimicrobial Peptide Transcripts. P. Gopalakrishnakone Editor-in-Chief; Gerardo Corzo, Maria Elena de Lima, Elia Diego-García, Editors. Spider Venoms. Dordrecht (Holanda). Springer Netherlands. pp. 224-249.
Periodo de realización: 1900/01/01 al 2016/01/01
Tipo: Capítulo de libro de divulgación
Lugar(es) de estudio: 2431 Veerle, Bélgica, Cuernavaca, Mor., México
Resumen: "The technological transformations that expand our knowledge of molecular biology in the 1980s brought
us various novel techniques and methods for gene isolation and characterization. Research groups from all
over the world began publishing the first scientific reports concerning transcripts and genes of several
spider species. Sophisticated techniques and methods for specific and random cDNA library screening
and the discovery of several expressed sequence tags (ESTs) enabled transcriptome analysis, opening up
new paths for investigation of poisonous and venomous animals and their venom components.
The transcriptomics allowed to report novel spider peptide toxin sequences, an important scientific
advancement that arrived together with several new scientific protagonists interested in exploring novel
venom compounds, such as proteomics. Even though the effort in the search and research of spider venom
components, transcripts and genes, has been significant, it has been lower compared to the total number of
molecules that is thought to be present in the spider venom glands according to a conservative estimate of
>9 million bioactive peptides (ca. 45 thousand spider species with 200 components per venom).
This chapter addresses the transcriptome analysis in spider venom glands using Sanger and nextgeneration
sequencing approaches. The emphasis is put on transcripts that encode for expressed peptide
toxins, which affect ion channels and expressed peptide toxins, which act as antimicrobial agents. This
manuscript aims to provide general information to strengthen the knowledge on the diversity of transcripts,
gene families, and the research of expressed spider compounds derived from their venom glands."