Spider Transcriptomes from Venom Glands: Molecular Diversity of Ion Channel Toxins and Antimicrobial Peptide Transcripts
Cita: Diego, E., Camila Takeno Cologna, Juliana Silva Cassoli, Gerardo A. Corzo. 2016. Spider Transcriptomes from Venom Glands: Molecular Diversity of Ion Channel Toxins and Antimicrobial Peptide Transcripts. P. Gopalakrishnakone Editor-in-Chief; Gerardo Corzo, Maria Elena de Lima, Elia Diego-García, Editors. Spider Venoms. Dordrecht (Holanda). Springer Netherlands. pp. 224-249.
Periodo de realización: 1900/01/01 al 2016/01/01
Tipo: Capítulo de libro de divulgación
Lugar(es) de estudio: 2431 Veerle, Bélgica, Cuernavaca, Mor., México
Resumen: "The technological transformations that expand our knowledge of molecular biology in the 1980s broughtus various novel techniques and methods for gene isolation and characterization. Research groups from allover the world began publishing the first scientific reports concerning transcripts and genes of severalspider species. Sophisticated techniques and methods for specific and random cDNA library screeningand the discovery of several expressed sequence tags (ESTs) enabled transcriptome analysis, opening upnew paths for investigation of poisonous and venomous animals and their venom components.The transcriptomics allowed to report novel spider peptide toxin sequences, an important scientificadvancement that arrived together with several new scientific protagonists interested in exploring novelvenom compounds, such as proteomics. Even though the effort in the search and research of spider venomcomponents, transcripts and genes, has been significant, it has been lower compared to the total number ofmolecules that is thought to be present in the spider venom glands according to a conservative estimate of>9 million bioactive peptides (ca. 45 thousand spider species with 200 components per venom).This chapter addresses the transcriptome analysis in spider venom glands using Sanger and nextgenerationsequencing approaches. The emphasis is put on transcripts that encode for expressed peptidetoxins, which affect ion channels and expressed peptide toxins, which act as antimicrobial agents. Thismanuscript aims to provide general information to strengthen the knowledge on the diversity of transcripts,gene families, and the research of expressed spider compounds derived from their venom glands."