Avanzada

Venom characterization of the bark scorpion Centruroides edwardsii (Gervais 1843): Composition, biochemical activities and in vivo toxicity for potential prey

Periodo de realización: 1900/01/01 al 2019/01/01

Tipo: Artículo científico

Lugar(es) de estudio: Tapachula de Córdova y Ordoñez, Chis., México, Lovaina, Bélgica, San José, Costa Rica
Resumen: "In this study, we characterize the venom of Centruroides edwardsii, one of the most abundant scorpions in urbanand rural areas of Costa Rica, in terms of its biochemical constituents and their biological activities. C. edwardsiivenom is rich in peptides but also contains some higher molecular weight protein components. No phospholipaseA2, hemolytic or fibrinogenolytic activities were found, but the presence of proteolytic and hyaluronidase enzymeswas evidenced by zymography.Venom proteomic analysis indicates the presence of a hyaluronidase, several cysteine-rich secretory proteins,metalloproteinases and a peptidylglycine a-hydroxylating monooxygenase like-enzyme. It also includes peptidessimilar to the Kþ-channel blocker margatoxin, a dominant toxin in the venom of the related scorpionC. margaritatus. MS and N-terminal sequencing analysis also reveals the presence of Naþ-channel-modulatingpeptides with sequence similarity to orthologs present in other scorpion species of the genera Centruroides andTityus. We purified the hyaluronidase (which co-eluted with an allergen 5-like CRiSP) and sequenced ~60% ofthis enzyme. We also sequenced some venom gland transcripts that include other cysteine-containing peptidesand a Non-Disulfide Bridged Peptide (NDBP).Our in vivo experiments characterizing the effects on potential predators and prey show that C. edwardsiivenom induces paralysis in several species of arthropods and geckos"

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