Bacillus subtilis with endocellulase and exocellulase activities isolated in the thermophilic phase from composting with coffee residues

Periodo de realización: 2017/06/01 al 2017/01/01

Tipo: Artículo científico

Lugar(es) de estudio: Tapachula, Chis., México
Resumen: "Abstract The goal of this study was to isolate, select and characterize bacteria with cellulolytic activity from two different coffee residue composting piles, one of which had an internal temperature of 57 ?C and pH 5.5 and the other, a temperature of 61 ?C, and pH 9.3. Culture media were manipulated with carboxymethylcellulose and crystalline cellulose as sole carbon sources. The enzyme activity was assessed by hydrolysis halo formation, reducing sugar production and zymograms. Three out of twenty isolated strains showed higher enzymatic activity and were identified as Bacillus subtilis according to their morphological, physiological, biochemical characteristics and based on the sequence analysis of 16S rDNA regions. The enzymatic extracts of the three selected strains showed exocellulase and endocellulase maximum activity of 0.254 and 0.519 U/ml, respectively"

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