Cellulases and xylanases production by Penicillium citrinum cgetcr using coffee pulp in solid state fermentation

Periodo de realización: 2017/06/01 al 2017/01/01

Tipo: Artículo científico

Lugar(es) de estudio: Tapachula, Chis., México
Resumen: "This work reports, for the first time, the use of co ee pulp residue to the expression of cellulolytic and xylanolytic enzymes by the native fungus Penicillium citrinum CGETCR by solid-state fermentation (SSF). Co ee is one of the most important drinks in the world producing several residues such as co ee pulp, which is considered a contaminant if not properly disposed. This residue causes damage to the fauna from rivers due to its composition including tannins, pH 3.5, (1-4%) ca eine, polyphenolic compound, ochratoxin A, the presence of the latter three have toxic or nephrotoxic e ects on higher animals. For these reasons, a study on the use of these residues to obtain high-added value products as enzymes of biotechnological interest, was performed. The enzyme production was optimized using the response surface methodology in a composite central design to study pH, moisture and fermentation time using endoglucanase activity as the response variable. The enzymatic activities found were - glucosidases, endoglucanases, exoglucanases and xylanases which were able to function under acidic (4.0-5.5) and basic (7.5-8.0) conditions. Additionally, protein extract was used on agroindustrial residues as substrate to evaluate reducing sugar production."

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