Cellulases and xylanases production by Penicillium citrinum cgetcr using coffee pulp in solid state fermentation
Periodo de realización: 2017/06/01 al 2017/01/01
Tipo: Artículo científico
Lugar(es) de estudio: Tapachula, Chis., México
Resumen: "This work reports, for the first time, the use of coee pulp residue to the expression of cellulolytic and xylanolytic enzymes by
the native fungus Penicillium citrinum CGETCR by solid-state fermentation (SSF). Coee is one of the most important drinks
in the world producing several residues such as coee pulp, which is considered a contaminant if not properly disposed. This
residue causes damage to the fauna from rivers due to its composition including tannins, pH 3.5, (1-4%) caeine, polyphenolic
compound, ochratoxin A, the presence of the latter three have toxic or nephrotoxic eects on higher animals. For these reasons,
a study on the use of these residues to obtain high-added value products as enzymes of biotechnological interest, was performed.
The enzyme production was optimized using the response surface methodology in a composite central design to study pH,
moisture and fermentation time using endoglucanase activity as the response variable. The enzymatic activities found were -
glucosidases, endoglucanases, exoglucanases and xylanases which were able to function under acidic (4.0-5.5) and basic (7.5-8.0)
conditions. Additionally, protein extract was used on agroindustrial residues as substrate to evaluate reducing sugar production."